{"id":592,"date":"2009-04-30T00:37:02","date_gmt":"2009-04-29T19:07:02","guid":{"rendered":"https:\/\/www.kashvet.org\/oasis\/?p=592"},"modified":"2009-06-03T00:26:54","modified_gmt":"2009-06-02T18:56:54","slug":"a-liquid-nitrogen-production-unit-preserving-life-for-life","status":"publish","type":"post","link":"https:\/\/www.kashvet.org\/oasis\/general\/a-liquid-nitrogen-production-unit-preserving-life-for-life\/","title":{"rendered":"A Liquid Nitrogen Production Unit &#8211; Preserving life for life"},"content":{"rendered":"<p>Liquid Nitrogen LN2 offers a effective mechanism of preserving semen at an extremely low temperature (-196 degrees centigrade). It is believed that freezing semen to this temperature would allow its preservation for decades if not centuries. The semen obtained is diluted wth various semen diluters (mainly egg yolk-buffer-glycerol based) and packed in small tubes (0.25 to 0.5 ml). These are then cooled and frozen in stages till they are finally dipped in liquid nitrogen and kept there in canisters. At the time of use they are simply thawed by placing in water bath at 37 degree celcius for 30 seconds.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-593\" title=\"LN2 plant liquid nitrogen plant and canister ba 32\" src=\"https:\/\/www.kashvet.org\/oasis\/wp-content\/uploads\/2009\/05\/cimg0910.jpg\" alt=\"LN2 plant liquid nitrogen plant and canister ba 32\" width=\"554\" height=\"416\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-594\" title=\"LN2 plant liquid nitrogen plant and canister ba 32\" src=\"https:\/\/www.kashvet.org\/oasis\/wp-content\/uploads\/2009\/05\/cimg0915.jpg\" alt=\"LN2 plant liquid nitrogen plant and canister ba 32\" width=\"554\" height=\"416\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-595\" title=\"LN2 plant liquid nitrogen plant and canister ba 32\" src=\"https:\/\/www.kashvet.org\/oasis\/wp-content\/uploads\/2009\/05\/cimg0916.jpg\" alt=\"LN2 plant liquid nitrogen plant and canister ba 32\" width=\"554\" height=\"416\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-596\" title=\"LN2 plant liquid nitrogen plant and canister ba 32\" src=\"https:\/\/www.kashvet.org\/oasis\/wp-content\/uploads\/2009\/05\/cimg0924.jpg\" alt=\"LN2 plant liquid nitrogen plant and canister ba 32\" width=\"554\" height=\"416\" \/><\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Liquid Nitrogen LN2 offers a effective mechanism of preserving semen at an extremely low temperature (-196 degrees centigrade). It is believed that freezing semen to this temperature would allow its preservation for decades if not centuries. The semen obtained is diluted wth various semen diluters (mainly egg yolk-buffer-glycerol based) and packed in small tubes (0.25 [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":25,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[228,522,521,523],"class_list":["post-592","post","type-post","status-publish","format-standard","hentry","category-general","tag-ai","tag-frozen-semen","tag-liquid-nitrogen","tag-plant"],"_links":{"self":[{"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/posts\/592","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/comments?post=592"}],"version-history":[{"count":5,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/posts\/592\/revisions"}],"predecessor-version":[{"id":600,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/posts\/592\/revisions\/600"}],"wp:attachment":[{"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/media?parent=592"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/categories?post=592"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kashvet.org\/oasis\/wp-json\/wp\/v2\/tags?post=592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}